Hotspot

trnR-ACG-trnN-GUU

IRb · 111181-111857 · 677 bp

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Pi 0.00197

MarkerSeek score 60.00

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 677
variable_sites: 2
pi: 0.00197
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	782 bp	1.0	77.3
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	769 bp	1.0	77.2
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TAGAATCTCCCGCTGTTCCT	55.0	50.0	57.8	57.8	827 bp	1.0	77.2
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	57.8	57.8	770 bp	1.0	76.8
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.7	57.8	819 bp	1.0	76.7