Hotspot

psbE-petL

LSC · 66757-68163 · 1407 bp

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Pi 0.00241

MarkerSeek score 61.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1407
variable_sites: 5
pi: 0.00241
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCCGAGGTTATAGTTAAAGCC	50.0	45.5	57.9	57.9	1546-1571 bp	1.0	64.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	CGAGGTTATAGTTAAAGCCGC	50.0	47.6	57.9	56.9	1543-1568 bp	1.0	64.1
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGCCGAGGTTATAGTTAAAGCC	50.0	45.5	57.8	57.9	1469-1494 bp	1.0	63.8
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AGCCGAGGTTATAGTTAAAGCC	45.0	45.5	58.2	57.9	1555-1580 bp	1.0	63.6
psbE-petL_p5	TGCTGACGAATAACCAACCC	AGCCGAGGTTATAGTTAAAGCC	50.0	45.5	58.2	57.9	1560-1585 bp	1.0	63.5