Hotspot

petA-psbJ

LSC · 65191-65964 · 774 bp

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Pi 0.000871

MarkerSeek score 60.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 1
pi: 0.000871
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGATTGGGTTCGTCGTTGTA	50.0	50.0	58.0	57.9	904-914 bp	1.0	80.0
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	GGATTGGGTTCGTCGTTGTA	50.0	50.0	58.2	57.9	901-911 bp	1.0	78.9
petA-psbJ_p3	GCATCTGTTGTTTTGGCACA	GGATTGGGTTCGTCGTTGTA	45.0	50.0	57.8	57.9	869-879 bp	1.0	78.6
petA-psbJ_p4	TTGGCATCTGTTGTTTTGGC	GGATTGGGTTCGTCGTTGTA	45.0	50.0	57.8	57.9	872-882 bp	1.0	78.4
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	GGATTGGGTTCGTCGTTGTA	45.0	50.0	57.6	57.9	899-909 bp	1.0	77.5