Hotspot

psbM-trnD-GUC

LSC · 30579-31331 · 753 bp

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Pi 0.000885
MarkerSeek score 59.50
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
753
variable_sites
1
pi
0.000885
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.8 58.5 864-1092 bp 1.0 58.5
psbM-trnD-GUC_p2 AGGAACTAGAATGAACAGTGCA TTCAATTGGTCAGAGCACCG 40.9 50.0 57.4 58.5 871-1099 bp 1.0 55.3
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC GTTCAATTGGTCAGAGCACC 45.0 50.0 55.8 57.0 865-1093 bp 1.0 54.3
psbM-trnD-GUC_p4 GGAACTAGAATGAACAGTGCAG TTCAATTGGTCAGAGCACCG 45.5 50.0 57.1 58.5 870-1098 bp 1.0 52.9
psbM-trnD-GUC_p5 AGAATGAACAGTGCAGTAGC TCAATTGGTCAGAGCACCG 45.0 52.6 55.8 57.8 863-1091 bp 1.0 52.5