Hotspot

atpH-atpI

LSC · 14079-15331 · 1253 bp

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Pi 0.00215

MarkerSeek score 61.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1253
variable_sites: 4
pi: 0.00215
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	AGTGGTTCCGATACCTGTCA	50.0	50.0	58.0	58.1	1424-1434 bp	1.0	80.2
atpH-atpI_p2	AATAGAAGCAAGCCCGACAG	AAGCGGCATTCAAGCTCTTA	50.0	45.0	58.0	57.9	1385-1395 bp	1.0	79.8
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1364-1374 bp	1.0	79.7
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	AGTGGTTCCGATACCTGTCA	50.0	50.0	57.9	58.1	1446-1456 bp	1.0	79.5
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	AAGCGGCATTCAAGCTCTTA	50.0	45.0	57.9	57.9	1407-1417 bp	1.0	79.2