Hotspot

trnR-UCU-atpA

LSC · 10274-10488 · 215 bp

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Pi 0

MarkerSeek score 21.40

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGCAAAGCGTCCATTGTCTA	TGGAACGTTTTCTACTTCAGGA	45.0	40.9	57.8	57.3	325-360 bp	1.0	57.2
trnR-UCU-atpA_p2	AATTCGGAATGCAAAGCGTC	TGGAACGTTTTCTACTTCAGGA	45.0	40.9	57.7	57.3	334-369 bp	1.0	56.5
trnR-UCU-atpA_p3	AATGCAAAGCGTCCATTGTC	TGGAACGTTTTCTACTTCAGGA	45.0	40.9	57.6	57.3	327-362 bp	1.0	55.8
trnR-UCU-atpA_p4	GAATGCAAAGCGTCCATTGT	TGGAACGTTTTCTACTTCAGGA	45.0	40.9	57.6	57.3	328-363 bp	1.0	55.8
trnR-UCU-atpA_p5	GGAATGCAAAGCGTCCATTG	TGGAACGTTTTCTACTTCAGGA	50.0	40.9	58.4	57.3	329-364 bp	1.0	55.7