Hotspot

trnH-GUG-psbA

LSC · 78-795 · 718 bp

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Pi 0.00105
MarkerSeek score 61.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
718
variable_sites
1
pi
0.00105
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.88
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCACAGAAAACGAATCCGCT 55.0 50.0 58.1 58.8 722-809 bp 1.0 76.3
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCACAGAAAACGAATCCGCT 50.0 50.0 57.9 58.8 736-823 bp 1.0 76.3
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCACAGAAAACGAATCCGCT 50.0 50.0 57.9 58.8 735-822 bp 1.0 76.3
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCACAGAAAACGAATCCGCT 50.0 50.0 57.9 58.8 737-824 bp 1.0 76.3
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG ACGAATCCGCTAATGAAGGT 55.0 45.0 58.1 57.0 713-800 bp 1.0 75.2