Hotspot

rps16

LSC · 5141-6251 · 1111 bp

Back to Santalum

Pi 0.00851
MarkerSeek score 62.30
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1111
variable_sites
32
pi
0.00851
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 TGTTGTTATAGGACGTGGGA TGGGTACTAGTTAAAGCAATGACT 45.0 37.5 55.9 57.6 1309-1314 bp 0.9 37.3
rps16_p2 GACGTGGGATTTTAAACAAGCA TGGGTACTAGTTAAAGCAATGACT 40.9 37.5 57.8 57.6 1298-1303 bp 0.9 37.3
rps16_p3 TTGTTGTTATAGGACGTGGGA TGGGTACTAGTTAAAGCAATGACT 42.9 37.5 56.7 57.6 1310-1315 bp 0.9 37.3
rps16_p4 ACGTGGGATTTTAAACAAGCA TGGGTACTAGTTAAAGCAATGACT 38.1 37.5 56.6 57.6 1297-1302 bp 0.9 37.3
rps16_p5 GGACGTGGGATTTTAAACAAGC TGGGTACTAGTTAAAGCAATGACT 45.5 37.5 58.4 57.6 1299-1304 bp 0.9 37.3