Hotspot

trnR-ACG-rrn5S

IRa · 119580-119986 · 407 bp

Back to Santalum

Pi 0.00687
MarkerSeek score 57.20
Primer available yes
Label trnR-ACG-rrn5S

Feature Metadata

label_name
trnR-ACG-rrn5S
feature_type
igs
region
IRa
length_bp
407
variable_sites
9
pi
0.00687
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5S_p1 CCACGTGCTCTAATCCACTG GGTGACGATACTGTAGGGGA 55.0 55.0 58.4 57.9 476-491 bp 0.9 76.2
trnR-ACG-rrn5S_p2 CCACGTGCTCTAATCCACTG AATCCATCCCGAACTTGGTG 55.0 50.0 58.4 57.9 511-526 bp 0.9 75.4
trnR-ACG-rrn5S_p3 CCACGTGCTCTAATCCACTG ACCAATCCATCCCGAACTTG 55.0 50.0 58.4 57.9 514-529 bp 0.9 75.4
trnR-ACG-rrn5S_p4 CCACGTGCTCTAATCCACTG CAATCCATCCCGAACTTGGT 55.0 50.0 58.4 57.9 512-527 bp 0.9 75.4
trnR-ACG-rrn5S_p5 CCACGTGCTCTAATCCACTG CACCAATCCATCCCGAACTT 55.0 50.0 58.4 57.9 515-530 bp 0.9 75.4