Hotspot

trnN-GUU-trnR-ACG

SSC · 118989-119505 · 517 bp

Back to Santalum

Pi 0.0146
MarkerSeek score 66.00
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
SSC
length_bp
517
variable_sites
28
pi
0.0146
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 GCTGTTAACTGACTGGTCGT TATAGTGGGGCCTGTAGCTC 50.0 55.0 57.8 58.0 573-679 bp 0.9 80.6
trnN-GUU-trnR-ACG_p2 GCTGTTAACTGACTGGTCGT CTATAGTGGGGCCTGTAGCT 50.0 55.0 57.8 58.0 574-680 bp 0.9 80.6
trnN-GUU-trnR-ACG_p3 TGGTCGTAGGTTCGAATCCT TATAGTGGGGCCTGTAGCTC 50.0 55.0 58.2 58.0 560-666 bp 0.9 80.6
trnN-GUU-trnR-ACG_p4 TGGTCGTAGGTTCGAATCCT CTATAGTGGGGCCTGTAGCT 50.0 55.0 58.2 58.0 561-667 bp 0.9 80.6
trnN-GUU-trnR-ACG_p5 CTGGTCGTAGGTTCGAATCC TATAGTGGGGCCTGTAGCTC 55.0 55.0 57.8 58.0 561-667 bp 0.9 80.2