Hotspot

trnL-UAG-rpl32

SSC · 109280-110067 · 788 bp

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Pi 0.0147
MarkerSeek score 64.90
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
788
variable_sites
49
pi
0.0147
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGAGCAGCGTGTCTACCTAT GCTTTTTCGCTAGCAAAATCTC 50.0 40.9 57.9 56.9 828-887 bp 0.9 57.6
trnL-UAG-rpl32_p2 TCCTAAGAGCAGCGTGTCTA GCTTTTTCGCTAGCAAAATCTC 50.0 40.9 57.9 56.9 833-892 bp 0.9 57.1
trnL-UAG-rpl32_p3 AAGAGCAGCGTGTCTACCTA GCTTTTTCGCTAGCAAAATCTC 50.0 40.9 58.2 56.9 829-888 bp 0.9 56.8
trnL-UAG-rpl32_p4 TAAGAGCAGCGTGTCTACCT GCTTTTTCGCTAGCAAAATCTC 50.0 40.9 58.2 56.9 830-889 bp 0.9 56.8
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC GCTTTTTCGCTAGCAAAATCTC 55.0 40.9 57.8 56.9 831-890 bp 0.9 56.5