Hotspot

trnP-UGG-psaJ

LSC · 66482-66851 · 370 bp

Back to Santalum

Pi 0.00775

MarkerSeek score 39.90

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 370
variable_sites: 8
pi: 0.00775
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	CCGGTGCTGTGGAAAGATAA	45.0	50.0	57.9	57.9	442-463 bp	1.0	79.6
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	CCCGCTAAAGATCCAAACCA	45.0	50.0	57.9	57.9	476-497 bp	1.0	79.4
trnP-UGG-psaJ_p3	AAAACAAACGCGCTACCAAG	ACCCGCTAAAGATCCAAACC	45.0	50.0	57.9	57.9	477-498 bp	1.0	79.4
trnP-UGG-psaJ_p4	CAAAACAAACGCGCTACCAA	CCGGTGCTGTGGAAAGATAA	45.0	50.0	58.2	57.9	443-464 bp	1.0	79.2
trnP-UGG-psaJ_p5	CAAAACAAACGCGCTACCAA	CCCGCTAAAGATCCAAACCA	45.0	50.0	58.2	57.9	477-498 bp	1.0	79.0