Hotspot

trnY-GUA-trnE-UUC

LSC · 32017-32075 · 59 bp

Back to Santalum

Pi 0.0169

MarkerSeek score 57.60

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 5
pi: 0.0169
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	ATCTCAGGATGGATCAGCGA	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.0	57.9	247 bp	1.0	80.8
trnY-GUA-trnE-UUC_p2	ATCTCAGGATGGATCAGCGA	TAAGTATGCCCCCATCGTCT	50.0	50.0	58.0	57.9	248 bp	1.0	80.8
trnY-GUA-trnE-UUC_p3	ATCTCAGGATGGATCAGCGA	TAGCGGTTGAGGACATCTCT	50.0	50.0	58.0	57.9	229 bp	1.0	80.5
trnY-GUA-trnE-UUC_p4	ATCTCAGGATGGATCAGCGA	TCTAGCGGTTGAGGACATCT	50.0	50.0	58.0	57.9	231 bp	1.0	80.5
trnY-GUA-trnE-UUC_p5	ATCTCAGGATGGATCAGCGA	GTAAGTATGCCCCCATCGTC	50.0	55.0	58.0	57.8	249 bp	1.0	80.2