Hotspot

trnH-GUG-psbA

LSC · 81-447 · 367 bp

Back to Santalum

Pi 0.0229

MarkerSeek score 75.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 29
pi: 0.0229
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CAATCCACTGCCTTGATCCA	CTGTTGAAGTTCCGTCCACA	50.0	50.0	57.9	58.1	349-447 bp	1.0	87.9
trnH-GUG-psbA_p2	AATCCACTGCCTTGATCCAC	CTGTTGAAGTTCCGTCCACA	50.0	50.0	57.9	58.1	348-446 bp	1.0	87.9
trnH-GUG-psbA_p3	ACAATCCACTGCCTTGATCC	CTGTTGAAGTTCCGTCCACA	50.0	50.0	57.9	58.1	350-448 bp	1.0	87.9
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	TAACCGTGCTAACCTGGGTA	50.0	50.0	57.9	58.1	420-518 bp	1.0	87.1
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	TAACCGTGCTAACCTGGGTA	50.0	50.0	57.9	58.1	419-517 bp	1.0	87.1