Hotspot

ycf1

SSC · 123635-129112 · 5478 bp

Back to Sansevieria

Pi 0.0028

MarkerSeek score 32.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5478
variable_sites: 28
pi: 0.0028
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATTGGTATGATCCACGGG	ATACGCATATCGGCACCAAG	50.0	50.0	57.1	58.2	267 bp	1.0	71.8
ycf1_p2	GATTGGTATGATCCACGGGT	ATACGCATATCGGCACCAAG	50.0	50.0	57.1	58.2	266 bp	1.0	71.8
ycf1_p3	TGATTGGTATGATCCACGGG	AATACGCATATCGGCACCAA	50.0	45.0	57.1	57.8	268 bp	1.0	71.5
ycf1_p4	GATTGGTATGATCCACGGGT	AATACGCATATCGGCACCAA	50.0	45.0	57.1	57.8	267 bp	1.0	71.5
ycf1_p5	TTGATTGGTATGATCCACGGG	ATACGCATATCGGCACCAAG	47.6	50.0	57.8	58.2	268 bp	1.0	69.6