Hotspot

ccsA

SSC · 114149-115120 · 972 bp

Back to Sansevieria

Pi 0.00171

MarkerSeek score 38.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 3
pi: 0.00171
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTGGCGGCATACTGTAAA	ACGACGGTCTTATGTATTCCT	50.0	42.9	58.0	56.3	1094 bp	1.0	58.3
ccsA_p2	GAGTGGCGGCATACTGTAAA	ACGACGGTCTTATGTATTCCTT	50.0	40.9	58.0	57.0	1094 bp	1.0	56.1
ccsA_p3	AGTGGCGGCATACTGTAAAA	ACGACGGTCTTATGTATTCCT	45.0	42.9	57.5	56.3	1093 bp	1.0	54.6
ccsA_p4	GAGTGGCGGCATACTGTAAA	ACGACGGTCTTATGTATTCC	50.0	45.0	58.0	54.7	1094 bp	1.0	53.8
ccsA_p5	GAGTGGCGGCATACTGTAAA	GTACGACGGTCTTATGTATTCCT	50.0	43.5	58.0	57.6	1096 bp	1.0	52.6