Hotspot

ndhC-trnV-UAC

LSC · 50159-51849 · 1691 bp

Back to Sansevieria

Pi 0.00386
MarkerSeek score 52.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1691
variable_sites
12
pi
0.00386
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCAAGATAGGAATAGCACCCG AGAAGGTCTACGGTTCGAGT 52.4 50.0 58.0 57.8 1780-1794 bp 1.0 71.2
ndhC-trnV-UAC_p2 TGGTCCTTCATTAATCGGAGC AGAAGGTCTACGGTTCGAGT 47.6 50.0 57.8 57.8 1824-1838 bp 1.0 70.0
ndhC-trnV-UAC_p3 GCCAAGATAGGAATAGCACCC AGAAGGTCTACGGTTCGAGT 52.4 50.0 58.2 57.8 1781-1795 bp 1.0 69.9
ndhC-trnV-UAC_p4 CCAAGATAGGAATAGCACCCG AGCCCAATGCAACAAAATGC 52.4 45.0 58.0 58.5 1862-1876 bp 1.0 69.0
ndhC-trnV-UAC_p5 CCAAGATAGGAATAGCACCCG GAAGGTCTACGGTTCGAGTC 52.4 55.0 58.0 57.5 1779-1793 bp 1.0 68.5