Pi
0.00735
MarkerSeek score
44.20
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AGAGAAAAAGCCGGCTATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 957-1000 bp | 1.0 | 80.9 |
| trnT-UGU-trnL-UAA_p2 | CGATAGAGAAAAAGCCGGCT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 961-1004 bp | 1.0 | 80.9 |
| trnT-UGU-trnL-UAA_p3 | TCGATAGAGAAAAAGCCGGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 962-1005 bp | 1.0 | 80.8 |
| trnT-UGU-trnL-UAA_p4 | AGAGAAAAAGCCGGCTATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 965-1008 bp | 1.0 | 80.4 |
| trnT-UGU-trnL-UAA_p5 | CGATAGAGAAAAAGCCGGCT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 969-1012 bp | 1.0 | 80.3 |