Hotspot

rps14-psaB

LSC · 36707-36843 · 137 bp

Back to Sansevieria

Pi 0.0109

MarkerSeek score 50.80

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 3
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	ATTTCTGCCGCTTCTTCTCC	TTGATTGCCTCCACATCAGG	50.0	50.0	58.3	57.9	216 bp	1.0	78.0
rps14-psaB_p2	CGACGGAACCCTGCTTATTT	TTGATTGCCTCCACATCAGG	50.0	50.0	58.3	57.9	278 bp	1.0	77.7
rps14-psaB_p3	ATTTCTGCCGCTTCTTCTCC	GCAGCTTTCTTGATTGCCTC	50.0	50.0	58.3	57.7	225 bp	1.0	76.8
rps14-psaB_p4	CGACGGAACCCTGCTTATTT	GCAGCTTTCTTGATTGCCTC	50.0	50.0	58.3	57.7	287 bp	1.0	76.5
rps14-psaB_p5	TTTCACTCAACGACGGAACC	TTGATTGCCTCCACATCAGG	50.0	50.0	58.4	57.9	288 bp	1.0	76.4