Hotspot

psbM-trnD-GUC

LSC · 29852-30550 · 699 bp

Back to Sansevieria

Pi 0.00334

MarkerSeek score 51.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 4
pi: 0.00334
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TGCTATACACCTTGCTGGGA	45.0	50.0	55.8	58.4	836-837 bp	1.0	59.3
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	810-811 bp	1.0	58.8
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	ACACCTTGCTGGGATTGTAG	45.0	50.0	55.8	57.5	830-831 bp	1.0	58.7
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	CACCTTGCTGGGATTGTAGT	45.0	50.0	55.8	57.5	829-830 bp	1.0	58.7
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	ATGCTATACACCTTGCTGGG	45.0	50.0	55.8	57.4	837-838 bp	1.0	57.5