Hotspot

atpH-atpI

LSC · 13824-14297 · 474 bp

Back to Sansevieria

Pi 0.00633

MarkerSeek score 52.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 474
variable_sites: 5
pi: 0.00633
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GGGGAATCCATAGAGGGTCA	45.0	55.0	58.0	57.9	533-534 bp	1.0	80.8
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	GGGGAATCCATAGAGGGTCA	50.0	55.0	58.1	57.9	621-622 bp	1.0	80.3
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	GGGGAATCCATAGAGGGTCA	50.0	55.0	57.9	57.9	589-590 bp	1.0	80.1
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	GGGGAATCCATAGAGGGTCA	45.0	55.0	58.2	57.9	534-535 bp	1.0	79.7
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	GGGGAATCCATAGAGGGTCA	55.0	55.0	58.2	57.9	593-594 bp	1.0	79.4