Hotspot

rps16-trnQ-UUG

LSC · 5678-6518 · 841 bp

Back to Sansevieria

Pi 0.00881
MarkerSeek score 53.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
841
variable_sites
14
pi
0.00881
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 916-925 bp 1.0 79.8
rps16-trnQ-UUG_p2 ATCATGTCCTTCAAGTCGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.2 58.1 917-926 bp 1.0 75.2
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.2 57.3 920-929 bp 1.0 75.0
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC TAAGGCAACGGGTTTTGGTC 50.0 50.0 58.2 58.7 946-955 bp 1.0 74.9
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC AAAACCTAAATGGGGCGTGG 50.0 50.0 58.2 58.7 974-983 bp 1.0 74.4