Hotspot

rps15

SSC · 125330-125602 · 273 bp

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Pi 0.00265

MarkerSeek score 25.40

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 5
pi: 0.00265
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GGTGCTGAGGACCCATATTG	CCATCCCCCTTTTTAACCCC	55.0	55.0	58.0	58.1	626-627 bp	0.666667	67.0
rps15_p2	GGTGCTGAGGACCCATATTG	AACCCCGCTCGAATCAAATT	55.0	45.0	58.0	58.2	612-613 bp	0.666667	66.7
rps15_p3	TGAGGTAAATCAGCAGCGAC	CCATCCCCCTTTTTAACCCC	50.0	55.0	58.0	58.1	1074-1075 bp	0.666667	66.6
rps15_p4	ATGAGTCGAAGAACACCGTG	CCATCCCCCTTTTTAACCCC	50.0	55.0	57.9	58.1	654-655 bp	0.666667	66.6
rps15_p5	TGAGGTAAATCAGCAGCGAC	AACCCCGCTCGAATCAAATT	50.0	45.0	58.0	58.2	1060-1061 bp	0.666667	66.3