Hotspot

rps15-ycf1

SSC · 125603-126003 · 401 bp

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Pi 0.0113

MarkerSeek score 60.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 401
variable_sites: 23
pi: 0.0113
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGACCCCTTGTTGTC	TGGAAGCCATTTCAGTATGT	50.0	40.0	57.7	54.6	497-522 bp	1.0	57.9
rps15-ycf1_p2	CAACAGACCCCTTGTTGTCT	TGGAAGCCATTTCAGTATGT	50.0	40.0	57.7	54.6	496-521 bp	1.0	57.9
rps15-ycf1_p3	TCAACAGACCCCTTGTTGTC	TGGAAGCCATTTCAGTATGTT	50.0	38.1	57.7	55.5	497-522 bp	1.0	56.7
rps15-ycf1_p4	CAACAGACCCCTTGTTGTCT	TGGAAGCCATTTCAGTATGTT	50.0	38.1	57.7	55.5	496-521 bp	1.0	56.6
rps15-ycf1_p5	TCAACAGACCCCTTGTTGTC	ATGGAAGCCATTTCAGTATGT	50.0	38.1	57.7	55.2	498-523 bp	1.0	54.9