Hotspot

trnP(TGG)-psaJ

LSC · 68381-68794 · 414 bp

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Pi 0.00906

MarkerSeek score 61.30

Primer available yes

Label trnP(TGG)-psaJ

Feature Metadata

label_name: trnP(TGG)-psaJ
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 22
pi: 0.00906
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP(TGG)-psaJ_p1	CTCCCTCGTTACTGCTCAAC	TGTCAACGCATCTGGGAAAA	55.0	45.0	58.0	58.0	614-649 bp	1.0	85.5
trnP(TGG)-psaJ_p2	ACCCGTGACATTTTGTACCC	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	561-596 bp	1.0	85.2
trnP(TGG)-psaJ_p3	CCCGTGACATTTTGTACCCA	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	560-595 bp	1.0	85.2
trnP(TGG)-psaJ_p4	CGTTACTGCTCAACGGAGAA	TGTCAACGCATCTGGGAAAA	50.0	45.0	57.9	58.0	608-643 bp	1.0	85.0
trnP(TGG)-psaJ_p5	ACAGGATTTGAACCCGTGAC	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	572-607 bp	1.0	85.0