Hotspot

ycf3-trnS(GGA)

LSC · 45739-46617 · 879 bp

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Pi 0.00993

MarkerSeek score 57.50

Primer available yes

Label ycf3-trnS(GGA)

Feature Metadata

label_name: ycf3-trnS(GGA)
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 36
pi: 0.00993
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS(GGA)_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	933-972 bp	1.0	77.8
ycf3-trnS(GGA)_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	904-943 bp	1.0	77.3
ycf3-trnS(GGA)_p3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	954-993 bp	1.0	77.2
ycf3-trnS(GGA)_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	906-945 bp	1.0	76.7
ycf3-trnS(GGA)_p5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	934-973 bp	1.0	75.5