Hotspot

trnE(TTC)-psbD

LSC · 32287-33955 · 1669 bp

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Pi 0.00554

MarkerSeek score 61.80

Primer available yes

Label trnE(TTC)-psbD

Feature Metadata

label_name: trnE(TTC)-psbD
feature_type: igs
region: LSC
length_bp: 1669
variable_sites: 46
pi: 0.00554
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE(TTC)-psbD_p1	GCTGCCTCCTTGAAAGAGAG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.3	58.2	1408-1858 bp	1.0	82.0
trnE(TTC)-psbD_p2	GCTGCCTCCTTGAAAGAGAG	TCCCTCCGTAACCAGTCATC	55.0	55.0	58.3	58.5	1393-1843 bp	1.0	79.9
trnE(TTC)-psbD_p3	TTGTAGTACCCTACCCCCAG	ACAAAAACGAAACGGTCCCT	55.0	45.0	57.4	58.2	1444-1894 bp	1.0	79.7
trnE(TTC)-psbD_p4	AACCACTAGACGATAGGGGC	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.6	58.2	1380-1830 bp	1.0	79.5
trnE(TTC)-psbD_p5	GCTGCCTCCTTGAAAGAGAG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.3	57.6	1407-1857 bp	0.952381	78.9