Hotspot

petN-psbM

LSC · 30027-31076 · 1050 bp

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Pi 0.0084

MarkerSeek score 51.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1050
variable_sites: 41
pi: 0.0084
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTACTGGCTGGGTCTTGAA	TTTTCGTTTCGCCCTTCTCA	50.0	45.0	58.0	58.0	1976-2378 bp	1.0	85.9
petN-psbM_p2	TCTACTGGCTGGGTCTTGAA	CCTTTTTCGTTTCGCCCTTC	50.0	50.0	58.0	58.0	1979-2381 bp	1.0	85.9
petN-psbM_p3	TCTACTGGCTGGGTCTTGAA	TTTTGAGTGGGCTGGGAATC	50.0	50.0	58.0	58.1	1615-2007 bp	1.0	85.7
petN-psbM_p4	TCTACTGGCTGGGTCTTGAA	AGTGGGCTGGGAATCCTATT	50.0	50.0	58.0	58.1	1610-2002 bp	1.0	85.6
petN-psbM_p5	CACGACTTCCCGATTCCTTT	TTTTCGTTTCGCCCTTCTCA	50.0	45.0	57.9	58.0	2018-2420 bp	1.0	85.3