Hotspot

rpoB-trnC(GCA)

LSC · 27959-29180 · 1222 bp

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Pi 0.00991

MarkerSeek score 55.90

Primer available yes

Label rpoB-trnC(GCA)

Feature Metadata

label_name: rpoB-trnC(GCA)
feature_type: igs
region: LSC
length_bp: 1222
variable_sites: 58
pi: 0.00991
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC(GCA)_p1	CGAGCCCATATCCTTGCTTT	TTCAAGACCCAGCCAGTAGA	50.0	50.0	58.0	58.0	1977-2048 bp	1.0	86.2
rpoB-trnC(GCA)_p2	TTGTTTCCTACTCACGCGAG	TTCAAGACCCAGCCAGTAGA	50.0	50.0	57.9	58.0	1993-2064 bp	1.0	85.9
rpoB-trnC(GCA)_p3	CCGCCTACACAAGCAAATTG	TTCAAGACCCAGCCAGTAGA	50.0	50.0	58.0	58.0	2169-2240 bp	1.0	85.7
rpoB-trnC(GCA)_p4	CTCATTTCCGTCTCGAAGCA	CTTACCACTCGGCCATATCG	50.0	55.0	58.0	57.9	1218-1275 bp	0.666667	70.3
rpoB-trnC(GCA)_p5	CTCATTTCCGTCTCGAAGCA	TTCAAGACCCAGCCAGTAGA	50.0	50.0	58.0	58.0	1492-1549 bp	0.666667	70.1