Hotspot

trnG(TCC)-trnR(TCT)

LSC · 10550-10764 · 215 bp

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Pi 0.021

MarkerSeek score 60.30

Primer available yes

Label trnG(TCC)-trnR(TCT)

Feature Metadata

label_name: trnG(TCC)-trnR(TCT)
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 24
pi: 0.021
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG(TCC)-trnR(TCT)_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	249-298 bp	1.0	74.1
trnG(TCC)-trnR(TCT)_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	252-301 bp	1.0	73.8
trnG(TCC)-trnR(TCT)_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	255-304 bp	1.0	73.3
trnG(TCC)-trnR(TCT)_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	258-307 bp	1.0	73.0
trnG(TCC)-trnR(TCT)_p5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.7	56.9	253-302 bp	1.0	71.5