Hotspot

trnS(GCT)-trnG(TCC)

LSC · 9067-9795 · 729 bp

Back to Sanicula

Pi 0.0118

MarkerSeek score 60.30

Primer available yes

Label trnS(GCT)-trnG(TCC)

Feature Metadata

label_name: trnS(GCT)-trnG(TCC)
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 42
pi: 0.0118
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(GCT)-trnG(TCC)_p1	ATTAGCAATCCGCCGCTTTA	AACCGAAAGACCCCTTAACT	45.0	45.0	58.0	56.4	713-851 bp	1.0	74.9
trnS(GCT)-trnG(TCC)_p2	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.2	56.4	762-900 bp	1.0	73.9
trnS(GCT)-trnG(TCC)_p3	ATTAGCAATCCGCCGCTTTA	AAACCGAAAGACCCCTTAACT	45.0	42.9	58.0	57.1	714-852 bp	1.0	73.0
trnS(GCT)-trnG(TCC)_p4	ACGGAAAGAGAGGGATTCGA	AAACCGAAAGACCCCTTAACT	50.0	42.9	58.2	57.1	763-901 bp	1.0	72.0
trnS(GCT)-trnG(TCC)_p5	GCTTTAGTCCACTCAGCCAT	AACCGAAAGACCCCTTAACT	50.0	45.0	57.6	56.4	699-837 bp	1.0	72.0