Hotspot

trnK(TTT)-rps16

LSC · 4350-5228 · 879 bp

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Pi 0.00613
MarkerSeek score 60.20
Primer available yes
Label trnK(TTT)-rps16

Feature Metadata

label_name
trnK(TTT)-rps16
feature_type
igs
region
LSC
length_bp
879
variable_sites
35
pi
0.00613
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK(TTT)-rps16_p1 TCCCTCGCTTCATCCAAATG CGGGAACTGTTCGGGATATT 50.0 50.0 58.0 57.4 994-1014 bp 1.0 79.6
trnK(TTT)-rps16_p2 CCGATGGTATGGACGAATCC CGGGAACTGTTCGGGATATT 55.0 50.0 57.9 57.4 1011-1031 bp 1.0 79.2
trnK(TTT)-rps16_p3 CGATGGTATGGACGAATCCC CGGGAACTGTTCGGGATATT 55.0 50.0 57.9 57.4 1010-1030 bp 1.0 79.2
trnK(TTT)-rps16_p4 AAAGCCGAGTACTCTACCGT CGGGAACTGTTCGGGATATT 50.0 50.0 58.2 57.4 950-970 bp 1.0 78.8
trnK(TTT)-rps16_p5 AAGCCGAGTACTCTACCGTT CGGGAACTGTTCGGGATATT 50.0 50.0 58.2 57.4 949-969 bp 1.0 78.8