Hotspot

ccsA-ndhD

SSC · 116866-117152 · 287 bp

Back to Sanguisorba

Pi 0.0486

MarkerSeek score 70.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 287
variable_sites: 43
pi: 0.0486
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCATTCTTTCGGGAGCAGTA	TTTTTGATTCTGGACCGCGA	50.0	45.0	58.0	58.1	656-720 bp	1.0	90.1
ccsA-ndhD_p2	TGCAGCTCTTTTGTGTGGAT	TTTTTGATTCTGGACCGCGA	45.0	45.0	58.0	58.1	922-986 bp	1.0	89.7
ccsA-ndhD_p3	ATGCAGCTCTTTTGTGTGGA	TTTTTGATTCTGGACCGCGA	45.0	45.0	58.0	58.1	923-987 bp	1.0	89.6
ccsA-ndhD_p4	CTGCAATTGTGGCCTCAATG	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	413-477 bp	0.833333	82.3
ccsA-ndhD_p5	CTGCAATTGTGGCCTCAATG	TTTTTGATTCTGGACCGCGA	50.0	45.0	58.0	58.1	509-573 bp	0.833333	81.6