Hotspot

rpl32-trnL-UAG

SSC · 115065-115734 · 670 bp

Back to Sanguisorba

Pi 0.0199
MarkerSeek score 68.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
670
variable_sites
68
pi
0.0199
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTCTTTCTACCGGTAGCTCA AGCGTGTCTACCGATTTCAC 47.6 50.0 57.6 58.0 720-754 bp 1.0 80.4
rpl32-trnL-UAG_p2 TCTCTTTCTACCGGTAGCTCA CAGCGTGTCTACCGATTTCA 47.6 50.0 57.6 58.0 721-755 bp 1.0 80.4
rpl32-trnL-UAG_p3 TCTCTTTCTACCGGTAGCTCA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.6 57.9 730-764 bp 1.0 79.4
rpl32-trnL-UAG_p4 TCTCTTTCTACCGGTAGCTCA CCTAAGAGCAGCGTGTCTAC 47.6 55.0 57.6 57.8 729-763 bp 1.0 78.8
rpl32-trnL-UAG_p5 TCTCTTTCTACCGGTAGCTCA CTAAGAGCAGCGTGTCTACC 47.6 55.0 57.6 57.8 728-762 bp 1.0 78.8