Hotspot

ndhF-rpl32

SSC · 113730-114905 · 1176 bp

Back to Sanguisorba

Pi 0.0199

MarkerSeek score 64.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1176
variable_sites: 107
pi: 0.0199
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAACGGT	AACTCGAGGTGCGCTTTTT	50.0	47.4	57.8	58.3	1075-1259 bp	1.0	79.6
ndhF-rpl32_p2	AGTTGCGGTCGGAAAAAGTA	AACTCGAGGTGCGCTTTTT	45.0	47.4	57.8	58.3	1122-1306 bp	1.0	79.0
ndhF-rpl32_p3	TAGTTGCGGTCGGAAAAAGT	AACTCGAGGTGCGCTTTTT	45.0	47.4	57.8	58.3	1123-1307 bp	1.0	79.0
ndhF-rpl32_p4	AAAGTAGAAGTCCCGCTCCT	AACTCGAGGTGCGCTTTTT	50.0	47.4	58.1	58.3	1189-1292 bp	0.916667	74.0
ndhF-rpl32_p5	AAAGTAGAAGTCCCGCTCCT	GAACTCGAGGTGCGCTTTTT	50.0	50.0	58.1	59.4	1190-1293 bp	0.916667	73.1