Hotspot

ycf1-ndhF

SSC · 111171-111485 · 315 bp

Back to Sanguisorba

Pi 0.249

MarkerSeek score 65.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 315
variable_sites: 109
pi: 0.249
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.35
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACGAAGGGGACTAAACAGGA	TCTCATATCCCCACGAGTCC	50.0	55.0	58.0	58.0	987-1235 bp	0.916667	88.5
ycf1-ndhF_p2	ACGAAGGGGACTAAACAGGA	GGGCGGTCGTATTTCTTCTT	50.0	50.0	58.0	58.0	440-688 bp	0.916667	88.3
ycf1-ndhF_p3	ACAGGAACAAGAGGGATCCA	TCTCATATCCCCACGAGTCC	50.0	55.0	58.0	58.0	973-1221 bp	0.916667	88.3
ycf1-ndhF_p4	GCAGAATACCGTCACCCATT	GGGCGGTCGTATTTCTTCTT	50.0	50.0	58.0	58.0	550-798 bp	0.916667	88.2
ycf1-ndhF_p5	GCAGAATACCGTCACCCATT	TCTCATATCCCCACGAGTCC	50.0	55.0	58.0	58.0	1097-1345 bp	0.916667	87.6