Hotspot

rpl22-rps19

LSC · 85234-85332 · 99 bp

Back to Sanguisorba

Pi 0.0532
MarkerSeek score 74.50
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
99
variable_sites
16
pi
0.0532
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.96
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 ATAACTCCTCCCTCGAGCTC GGGCCCCAATTGGTAGAAAA 55.0 50.0 58.0 58.1 860-928 bp 1.0 86.9
rpl22-rps19_p2 ATAACTCCTCCCTCGAGCTC TGGTAGGGCATAAGTTGGGA 55.0 50.0 58.0 58.0 486-539 bp 0.916667 84.0
rpl22-rps19_p3 GATAACTCCTCCCTCGAGCT TGGTAGGGCATAAGTTGGGA 55.0 50.0 58.0 58.0 487-540 bp 0.916667 84.0
rpl22-rps19_p4 ATAACTCCTCCCTCGAGCTC TCTCGGGCGTCTACCATTAT 55.0 50.0 58.0 58.0 580-633 bp 0.916667 82.4
rpl22-rps19_p5 GATAACTCCTCCCTCGAGCT TCTCGGGCGTCTACCATTAT 55.0 50.0 58.0 58.0 581-634 bp 0.916667 82.4