Hotspot

rps18-rpl20

LSC · 69467-69762 · 296 bp

Back to Sanguisorba

Pi 0.0124

MarkerSeek score 70.60

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 296
variable_sites: 17
pi: 0.0124
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	TGAAAGAAGCGAGTCAACCG	TGTACAAGAAACAGTTGCTTCT	50.0	36.4	58.5	56.2	432-454 bp	1.0	52.4
rps18-rpl20_p2	TGAAAGAAGCGAGTCAACCG	TGTACAAGAAACAGTTGCTTCTT	50.0	34.8	58.5	56.9	432-454 bp	1.0	50.0
rps18-rpl20_p3	CCGCTAGAACTACGGGTCTT	TGTACAAGAAACAGTTGCTTCT	55.0	36.4	58.9	56.2	415-437 bp	1.0	49.5
rps18-rpl20_p4	TGAAAGAAGCGAGTCAACCG	AGAAACAGTTGCTTCTTAATCGT	50.0	34.8	58.5	56.7	426-448 bp	1.0	49.2
rps18-rpl20_p5	AGTCAACCGCTAGAACTACG	TGTACAAGAAACAGTTGCTTCT	50.0	36.4	57.1	56.2	421-443 bp	0.916667	44.4