Hotspot

ndhC-trnV-UAC

LSC · 52234-52901 · 668 bp

Back to Sanguisorba

Pi 0.0368

MarkerSeek score 73.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 90
pi: 0.0368
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TTTTCCGGCCCTTTGCTAAT	AGAAGGTCTACGGTTCGAGT	45.0	50.0	58.1	57.8	683-826 bp	1.0	89.9
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	662-805 bp	1.0	89.3
ndhC-trnV-UAC_p3	TTTTTCCGGCCCTTTGCTAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	58.3	57.8	684-827 bp	1.0	88.1
ndhC-trnV-UAC_p4	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	674-817 bp	1.0	87.7
ndhC-trnV-UAC_p5	TTTTCCGGCCCTTTGCTAAT	GAAGGTCTACGGTTCGAGTC	45.0	55.0	58.1	57.5	682-825 bp	1.0	87.1