Hotspot

trnL-UAA-trnF-GAA

LSC · 49239-49652 · 414 bp

Back to Sanguisorba

Pi 0.0254

MarkerSeek score 68.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 36
pi: 0.0254
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	446-473 bp	1.0	86.5
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	482-509 bp	1.0	85.6
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	458-485 bp	1.0	85.3
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	497-524 bp	1.0	85.0
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	CATGTGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	500-527 bp	1.0	84.4