Hotspot

trnC-GCA-petN

LSC · 27561-28347 · 787 bp

Back to Sanguisorba

Pi 0.0228

MarkerSeek score 66.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 62
pi: 0.0228
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.1	58.0	818-851 bp	1.0	89.0
trnC-GCA-petN_p2	TGTATTGTTTTGGCGGCATG	CAGCCCAAGCGAGATTTACT	45.0	50.0	57.6	58.0	871-904 bp	1.0	86.4
trnC-GCA-petN_p3	GTATTGTTTTGGCGGCATGG	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.4	58.0	870-903 bp	1.0	86.4
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	878-911 bp	1.0	85.8
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.4	58.0	836-869 bp	0.916667	80.3