Hotspot

atpH-atpI

LSC · 13447-14191 · 745 bp

Back to Sanguisorba

Pi 0.0138

MarkerSeek score 70.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 45
pi: 0.0138
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGGGGAATCCATGGAAGGT	45.0	50.0	58.0	57.4	786-808 bp	1.0	83.3
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TAGGGGAATCCATGGAAGGT	45.0	50.0	58.2	57.4	787-809 bp	1.0	82.2
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACCCTAGCTGC	45.0	45.0	58.0	57.3	814-836 bp	1.0	82.0
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	AGGGGAATCCATGGAAGGTC	45.0	55.0	58.0	58.8	785-807 bp	1.0	82.0
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	ACAAGCGGTATTCAAGCTCT	45.0	45.0	58.0	57.2	835-857 bp	1.0	81.8