Hotspot

rps16-trnQ-UUG

LSC · 6284-7227 · 944 bp

Back to Sanguisorba

Pi 0.0235
MarkerSeek score 75.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
944
variable_sites
95
pi
0.0235
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 877-1088 bp 1.0 89.6
rps16-trnQ-UUG_p2 TCCACAGTCGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 899-1110 bp 1.0 89.4
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 888-1099 bp 1.0 89.0
rps16-trnQ-UUG_p4 CTCCACAGTCGATCATGTCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.8 58.1 900-1111 bp 1.0 88.9
rps16-trnQ-UUG_p5 CCACAGTCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.3 58.1 898-1109 bp 1.0 85.1