Hotspot

trnK-UUU-rps16

LSC · 4315-5113 · 799 bp

Back to Sanguisorba

Pi 0.0242
MarkerSeek score 73.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
799
variable_sites
89
pi
0.0242
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 903-970 bp 1.0 90.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 902-969 bp 1.0 90.3
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 912-979 bp 1.0 89.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.2 58.2 907-974 bp 1.0 88.6
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 906-973 bp 1.0 88.6