Hotspot

ycf1

SSC · 127900-133599 · 5700 bp

Back to Sambucus

Pi 0.00896

MarkerSeek score 47.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5700
variable_sites: 147
pi: 0.00896
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCATCATTACACAATCGAGTCCT	ATGCCAGAGTGATGGAAAGC	39.1	50.0	57.4	58.2	261 bp	1.0	53.3
ycf1_p2	TCATCATTACACAATCGAGTCCT	GCCAGAGTGATGGAAAGCAA	39.1	50.0	57.4	58.5	259 bp	1.0	51.5
ycf1_p3	TCATCATTACACAATCGAGTCC	ATGCCAGAGTGATGGAAAGC	40.9	50.0	56.0	58.2	261 bp	1.0	50.1
ycf1_p4	CATCATTACACAATCGAGTCCT	ATGCCAGAGTGATGGAAAGC	40.9	50.0	56.0	58.2	260 bp	1.0	50.0
ycf1_p5	TCATCATTACACAATCGAGTCCTT	ATGCCAGAGTGATGGAAAGC	37.5	50.0	58.0	58.2	261 bp	1.0	49.9