Hotspot

rpl32-trnL-UAG

SSC · 117286-118218 · 933 bp

Back to Sambucus

Pi 0.0108

MarkerSeek score 64.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 933
variable_sites: 30
pi: 0.0108
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTAAAAGCTTTGT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	905-1079 bp	1.0	85.3
rpl32-trnL-UAG_p2	GGCAGCGTTAAAAGCTTTGT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	906-1080 bp	1.0	85.3
rpl32-trnL-UAG_p3	AAAAGGAAGGGGTATTGGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.8	58.0	922-1096 bp	1.0	84.3
rpl32-trnL-UAG_p4	AAAAGGAAGGGGTATTGGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.8	58.0	923-1097 bp	1.0	84.3
rpl32-trnL-UAG_p5	GGCAGCGTTAAAAGCTTTGT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	915-1089 bp	1.0	84.3