Hotspot

nad5-rpl32

SSC · 115958-117120 · 1163 bp

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Pi 0.0119

MarkerSeek score 66.70

Primer available yes

Label nad5-rpl32

Feature Metadata

label_name: nad5-rpl32
feature_type: igs
region: SSC
length_bp: 1163
variable_sites: 44
pi: 0.0119
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
nad5-rpl32_p1	CTGGAAGTGGAAGGAAAGGT	ACAAAGCTTTTAACGCTGCC	50.0	45.0	57.0	57.9	1312-1418 bp	1.0	77.8
nad5-rpl32_p2	CTGGAAGTGGAAGGAAAGGT	GCCCAATACCCCTTCCTTTT	50.0	50.0	57.0	57.8	1295-1401 bp	1.0	77.0
nad5-rpl32_p3	CTGGAAGTGGAAGGAAAGGT	TTTAACGCTGCCCAATACCC	50.0	50.0	57.0	58.5	1304-1410 bp	1.0	74.6
nad5-rpl32_p4	CTGGAAGTGGAAGGAAAGGT	CAAAGCTTTTAACGCTGCCC	50.0	50.0	57.0	58.6	1311-1417 bp	1.0	74.3
nad5-rpl32_p5	CTGGAAGTGGAAGGAAAGGT	AGCTTTTAACGCTGCCCAAT	50.0	45.0	57.0	58.7	1308-1414 bp	1.0	73.0