Hotspot

trnN-GUU-nad5

IRb · 112159-113716 · 1558 bp

Back to Sambucus

Pi 0.0332

MarkerSeek score 88.70

Primer available yes

Label trnN-GUU-nad5

Feature Metadata

label_name: trnN-GUU-nad5
feature_type: igs
region: IRb
length_bp: 1558
variable_sites: 119
pi: 0.0332
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 88.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-nad5_p1	AGGATTCGAACCTACGACCA	CGGGCGCATTTCTTCATATC	50.0	50.0	58.2	57.4	1638-1718 bp	1.0	83.8
trnN-GUU-nad5_p2	CCCCAAGTAGGATTCGAACC	CGGGCGCATTTCTTCATATC	55.0	50.0	57.7	57.4	1646-1726 bp	1.0	82.4
trnN-GUU-nad5_p3	GGATTCGAACCTACGACCAA	CGGGCGCATTTCTTCATATC	50.0	50.0	57.3	57.4	1637-1717 bp	1.0	79.7
trnN-GUU-nad5_p4	AGGATTCGAACCTACGACCA	GGTATCAAATCTGTAGGGGGC	50.0	52.4	58.2	57.9	1658-1738 bp	1.0	79.4
trnN-GUU-nad5_p5	AGGATTCGAACCTACGACCA	TATCAAATCTGTAGGGGGCG	50.0	50.0	58.2	56.8	1656-1736 bp	1.0	78.8