Hotspot

psaA-ycf3

LSC · 43734-44473 · 740 bp

Back to Sambucus

Pi 0.00556

MarkerSeek score 63.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 13
pi: 0.00556
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	904-934 bp	1.0	82.9
psaA-ycf3_p2	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	922-952 bp	1.0	82.8
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	924-954 bp	1.0	82.0
psaA-ycf3_p4	TGAGAAATGACCCGGTTTGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	918-948 bp	1.0	81.1
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.1	58.3	920-950 bp	1.0	81.1