Hotspot

trnM-CAU-psbD

LSC · 33328-34756 · 1429 bp

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Pi 0.0186
MarkerSeek score 64.00
Primer available yes
Label trnM-CAU-psbD

Feature Metadata

label_name
trnM-CAU-psbD
feature_type
igs
region
LSC
length_bp
1429
variable_sites
68
pi
0.0186
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.94
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-psbD_p1 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.3 53.0 1209-1483 bp 1.0 47.8
trnM-CAU-psbD_p2 GGCGTAAGTCATCGGTTCAA TTACCAAGGGCTATAGTCAT 50.0 40.0 58.3 52.8 1208-1482 bp 1.0 47.8
trnM-CAU-psbD_p3 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCAT 50.0 38.1 58.3 53.7 1209-1483 bp 1.0 47.8
trnM-CAU-psbD_p4 CCCCTTTAACTCAGCGGTAG TTTACCAAGGGCTATAGTCA 55.0 40.0 57.7 53.0 1245-1519 bp 1.0 47.7
trnM-CAU-psbD_p5 CCCCTTTAACTCAGCGGTAG TTACCAAGGGCTATAGTCAT 55.0 40.0 57.7 52.8 1244-1518 bp 1.0 47.7